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An Article About Tissue Culture.

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Agar is one of those staples of the tissue culture industry we generally can not do without. Agar is a gelatin like substance which is derived from common seaweed. You can find it used in many old fashion and modern desserts such as those found in Japan and are known as anmitsu.

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Several people have written to me for information relating to fern spore cultures. I am not an expert at this phase of micropropagation and have only a vague familiarity with this type of cultured plants but here is what I do know. Hopefully it is of use to you.

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Experimenters are always on the look out for unique and unusual methods by which they can sterilize the particular media that they are employing for their tissue culture work. The time honored home lab process is by the use of a home pressure cooker. Although there is one easier and simpler method that is often overlooked – the use of the family microwave oven.

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Failing in tissue culture experiments is very easy if you are slack in your procedures and careless when you do your initial explants setups. Some of the major reasons that have been cited for unproductive cultures are as follows…

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I generally purchase my tissue culture containers by the case and it goes without saying that they can get rather expensive. The particular ones that I like to use are made out of plastic which tends to make them slightly more affordable then those created from pure glass.

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Standard procedure for dividing (and multiplying) cell lines grown in flasks or petri dishes.