Recombination technology begins with the isolation of a gene of interest. The gene is inserted into a vector and cloned. A vector is a piece of DNA that are capable of independent growth, vectors used are bacterial plasmids and viral Phags. The gene of interest (foreign DNA) is integrated into the plasmid or Phags, called recombinant DNA.
This is how recombination technology:
A. recombination technology begins with the isolation of a gene of interest. The gene is inserted into a vector and cloned. A vector is a piece of DNA that are capable of independent growth, vectors used are bacterial plasmids and viral Phags. The gene of interest (foreign DNA) is integrated into the plasmid or Phags, called recombinant DNA.
2. Before introducing the vector containing the foreign DNA into host cells to express the protein to be cloned. Cloning is necessary to produce many copies of DNA from the initial offer is sufficient to insert into host cells.
Three. When the vector is isolated in large quantities, can be brought into host cells such as yeast, like mammals, or special bacterial cells. The host cells and the synthesis of foreign proteins using recombinant DNA. When cells are grown in large quantities, or recombinant foreign proteins can be isolated and purified in large quantities.
Other use of recombinant DNA
Recombinant DNA technology is not only an important tool in scientific research, but has also affected the diagnosis and treatment of diseases and genetic disorders in several areas of medicine. A permit many advances, including:
* The isolation of large quantities of proteins
In addition to follicle-stimulating hormone (FSH) is used Follistim (follitropin beta injection) and Follistim AQ Vial (follitropin beta injection), insulin, growth hormone and other proteins are now available as recombinant products.
* Identification of mutations
People can be tested for the presence of mutated proteins that may be associated with breast cancer, retino blastoma, and neurofibromatosis.
* Diagnosis of the affected states and the carrier of hereditary diseases
There are tests to determine whether individuals are carriers of the cystic fibrosis gene, the gene for Huntington’s disease, the gene for Tay-Sachs, or the gene for Duchenne muscular dystrophy.
* The transfer of genes from one organism to another
People suffering from cystic fibrosis, rheumatoid arthritis, vascular disease and certain types of cancer can now benefit from advances in gene therapy.
Distribution of genes on human chromosomes
Scientists are able to link mutations and disease states to specific locations on chromosomes.